Research Protocols

This page outlines general best practices for handling, storing, and preparing research-grade peptides. These guidelines are for reference only and do not constitute medical advice. Researchers should follow their institution’s biosafety protocols and consult with their research supervisor.

Peptide Storage and Stability

Unopened Vials

  • Store at room temperature (15-25°C) away from direct sunlight
  • Keep away from moisture and humidity
  • Unopened vials typically remain stable for 2 years

Reconstituted Solutions

  • Use sterile, bacteriostatic water (0.9% benzyl alcohol in saline) for reconstitution
  • Store reconstituted peptides at 2-8°C (refrigerator)
  • Most reconstituted peptides remain stable for 2-4 weeks when refrigerated
  • For extended storage, freeze at -20°C for up to 6 months
  • Avoid repeated freeze-thaw cycles — divide into working aliquots if possible

Reconstitution Protocol

  1. Calculate volume needed: Determine the amount of bacteriostatic water required based on your desired final concentration. Refer to the product specifications.
  2. Prepare supplies: Gather sterile syringes (appropriate gauge for subcutaneous injection if applicable), alcohol prep pads, and bacteriostatic water. Ensure all materials are sterile and uncontaminated.
  3. Sanitize the vial: Wipe the rubber septum of the peptide vial with an alcohol prep pad.
  4. Inject water slowly: Using a sterile syringe, slowly inject the bacteriostatic water along the inner wall of the vial (not directly onto the powder) to minimize foaming and degradation.
  5. Allow dissolution time: Let the vial sit undisturbed for 10-15 minutes to allow the peptide to fully dissolve.
  6. Mix gently: Gently roll or swirl the vial between your palms to mix. Do not shake vigorously, as this can create bubbles and denature the peptide.
  7. Verify clarity: The solution should be clear and colorless (or the color appropriate to the specific peptide). If cloudiness persists, contact support.

Dosing and Administration

  • Use the Dose Calculator to determine precise injection volumes based on your reconstituted concentration and desired micrograms.
  • Use sterile syringes and sterile injection technique at all times.
  • Rotate injection sites to minimize localized irritation during repeat-dose research.
  • Document injection sites, times, and volumes in your research protocol.
  • Dispose of used needles and syringes in a sharps container or biohazard waste container per institutional guidelines.

Quality Control

  • Each Peptides.li product includes a Certificate of Analysis (CoA) from a third-party laboratory. Review this before use to confirm purity, identity, and absence of microbial contamination.
  • Store your CoA with your research records.
  • Report any visual abnormalities (discoloration, particles, cloudiness in reconstituted solution) immediately to support@peptides.li.

Safety and Biosafety

  • Handle all research compounds in compliance with your institution’s biosafety level (BSL) requirements.
  • Use appropriate personal protective equipment (gloves, lab coat, safety glasses).
  • Work in a clean, controlled environment to minimize contamination.
  • Follow your institution’s biological waste disposal protocols.
  • Never consume, inhale, or apply research peptides to yourself or others.

Research Ethics

All research involving peptides must be conducted in accordance with:

  • Your institution’s Institutional Review Board (IRB) or equivalent ethics committee approval
  • All applicable federal, state, and local laws and regulations
  • Your institution’s animal use committee (IACUC) if research involves animals
  • Good Laboratory Practice (GLP) and Standard Operating Procedures (SOPs) established by your institution